SNP discovery via 454 transcriptome sequencing
نویسندگان
چکیده
منابع مشابه
SNP discovery via 454 transcriptome sequencing
A massively parallel pyro-sequencing technology commercialized by 454 Life Sciences Corporation was used to sequence the transcriptomes of shoot apical meristems isolated from two inbred lines of maize using laser capture microdissection (LCM). A computational pipeline that uses the POLYBAYES polymorphism detection system was adapted for 454 ESTs and used to detect SNPs (single nucleotide polym...
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454 DNA sequencing technology achieves significant throughput relative to traditional approaches. More than 261,000 ESTs were generated by 454 Life Sciences from cDNA isolated using laser capture microdissection (LCM) from the developmentally important shoot apical meristem (SAM) of maize (Zea mays L.). This single sequencing run annotated >25,000 maize genomic sequences and also captured appro...
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ژورنال
عنوان ژورنال: The Plant Journal
سال: 2007
ISSN: 0960-7412,1365-313X
DOI: 10.1111/j.1365-313x.2007.03193.x